| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Kluyvera ascorbata | TCCGATGCAGTGGTACAGC | AACGTGCAGTGTACGGCA | 59.78 | 59.89 | 258 | 116 |
100.00%
|
50.00%
|
| Primer 22 | Kluyvera ascorbata | TCGTTTCTGGCGACGGTA | CCGTTGAGTTTGCGATCGC | 58.65 | 60.23 | 190 | 114 |
100.00%
|
50.00%
|
| Primer 23 | Kluyvera ascorbata | TGCTGCACTGCACCTTCT | AGCCAGGTCATCGCCTCTT | 59.49 | 60.99 | 197 | 114 |
100.00%
|
50.00%
|
| Primer 24 | Kluyvera ascorbata | TGCTGCACTGCACCTTCT | AGAAGCCAGGTCATCGCCT | 59.49 | 60.99 | 200 | 111 |
100.00%
|
50.00%
|
| Primer 25 | Kluyvera ascorbata | ACGCTATGTGCCGTTGGT | CTCCAGTTGACCCAGCGTT | 59.65 | 59.93 | 271 | 111 |
100.00%
|
50.00%
|
| Primer 26 | Kluyvera ascorbata | CGCAGCAGCAACATCACTC | TGCTCGCGTTTAGCCACT | 59.87 | 59.66 | 264 | 105 |
100.00%
|
50.00%
|
| Primer 27 | Kluyvera ascorbata | CGCAGCAGCAACATCACT | TGCTCGCGTTTAGCCACT | 58.74 | 59.66 | 264 | 105 |
100.00%
|
50.00%
|
| Primer 28 | Kluyvera ascorbata | TTCTGCTGCTGCCGAAGA | TGTTGCCAATCGCCAGCA | 59.26 | 60.60 | 183 | 103 |
100.00%
|
100.00%
|
| Primer 29 | Kluyvera ascorbata | AGCGATTGGCCTTGTGGT | AGAACAGCGTCGCGAACA | 59.56 | 59.97 | 155 | 95 |
100.00%
|
100.00%
|
| Primer 30 | Kluyvera ascorbata | TTGCCTGTGATGCTGCCA | TGCGACGGGTGCAAAGAA | 59.88 | 60.20 | 200 | 95 |
100.00%
|
50.00%
|